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. 2017 Jan 13;16:212–223. doi: 10.1016/j.ebiom.2017.01.015

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© 2017 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 6 — Loss of miR-133a alleviates the inhibitory effect of MyoD on brown adipogenesis. (a) ORO staining of brown adipocytes differentiated from miR-133a^−/− BAT SVF cells transduced with control or MyoD^OE adenovirus. (b and c) Immunostaining of BODIPY (red) in control (b) and MyoD^OE (c) miR-133a^−/− brown adipocytes. (d) Ratios of BODIPY signal in GFP⁺ cells in control and MyoD^OE miR-133a^−/− brown adipocytes. Five different areas analyzed in each experiment. (e) Fold changes of GFP⁺ BODIPY⁺ cells in WT and miR-133a^−/− brown adipocytes after MyoD overexpression (f) qPCR analysis of miR-133a and miR-133b. (g and h) Fold changes of myogenic markers (g) and adipogenic markers (h) in WT and miR-133a^−/− brown adipocytes after MyoD overexpression. Error bars represent mean with SD of three independent biological replicates.^⁎P < 0.05 (Student's t-test).