Fig. 2.

DLA-M decreases the fat accumulation in the adipose tissues and livers of HFD mice. Epi-WAT weight and Epi-WAT/BW ratio (a), Per-WAT weight and Per-WAT/BW ratio (b), and Mes-WAT weight and Mes-WAT/BW ratio (c) of the mice described in Fig. 1. (d) H&E staining of Epi-WAT sections from mice described in (a) (scale: 200 μm). Mean epididymal adipocyte size (e) and adipocyte size frequency (f) in each group (n = 5 mice per group). (g) Oil Red O staining of liver sections (scale: 100 μm) and liver TG content (h) of mice. (i) Liver mRNA expression of genes involved in lipogenesis was measured by real-time PCR analysis. (j) The FABP protein production (upper panel) and relative protein level (lower panel) were examined in the liver. Values are shown as the means ± s.e.m. *, P < 0.05; **, P < 0.01; ***, and P < 0.001 compared with NCD. #, P < 0.05; ##, P < 0.01; and ###, P < 0.001 compared with HFD by ANOVA with Tukey's multiple comparison test.