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. 2017 Jun 19;8:1129. doi: 10.3389/fmicb.2017.01129

FIGURE 2.

FIGURE 2

Abl is not involved in the replication stage of the HCV life cycle. (A) Huh7 cells harboring HCV subgenomic replicon derived from genotype 1b were transfected with the indicated siRNAs. At 3 days after transfection, total cell lysates were immunoblotted with the indicated antibodies. (B) Huh7.5 cells were infected with Jc1 for 4 h. At 48 h post-infection, cells were transfected with the indicated siRNAs. Total cell lysates harvested at 2 days after transfection were immunoblotted with the indicated antibodies. (C) Huh7.5 cells were treated as described in (B) and then intracellular RNA levels of both HCV and Abl were quantified by qRT-PCR. The asterisks indicate significant differences (∗P < 0.05; ∗∗P < 0.01). (D) Huh7 cells harboring HCV subgenomic replicon (genotype 1b) were either left untreated or treated with DMSO, or the indicated concentrations of imatinib (left panel) or dasatinib (right panel). At 72 h after inhibitor treatments, total cell lysates were immunoblotted with the indicated antibodies. (E) Huh7.5 cells were infected with Jc1 for 4 h. At 48 h post-infection, cells were left untreated or treated with either DMSO or the indicated concentrations of imatinib (left panel) or dasatinib (right panel). Forty-eight hours after inhibitor treatments, total cell lysates were immunoblotted with the indicated antibodies. (F) Huh7.5 cells were treated as described in (E) and then intracellular HCV RNA levels were quantified by qRT-PCR.