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. 2017 Jun 19;8:717. doi: 10.3389/fimmu.2017.00717

Figure 5.

Figure 5

Confirmatory flow cytometry results. Analyzed flow cytometry results for a selection of markers for 37 patients [n = 15, no chronic graft-versus-host disease (cGVHD); n = 7, mild cGVHD; n = 10, moderate cGVHD; and n = 5, severe cGVHD]. GzB, Granzyme B. Statistical analysis was done with the Mann–Whitney U test. (A) A potential NKT-cell subset, based on cluster 399954 (Figure 3C). Representative flow cytometry figures of a patient without cGVHD and with mild cGVHD are shown to the right of the graphs gated on T-cells. (B) CD38 expression on CD39+ CXCR5+ HLA-DR+ B-cells. Representative flow cytometry figures of a patient without cGVHD and with mild cGVHD are shown below the graphs. (C) CD39 expression on CXCR5+ HLA-DR+ B-cells. Representative flow cytometry figures of a patient with moderate and severe cGVHD are shown below the graphs. (D) CD8 expression on CD57+ GzB+ T-cells. Representative flow cytometry figures of a patient with moderate and severe cGVHD are shown below the graphs.