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. 2017 Jun 19;8:340. doi: 10.3389/fphar.2017.00340

FIGURE 4.

FIGURE 4

Effects of the metabolites naringenin-7/4′-O-glucuronide (Nge-7 or Nge-4′) and naringenin (Nge) on axonal regrowth after Aβ-induced axonal atrophy. Primary cortical neurons were cultured for 3 days, and then the cells were treated with 10 μM Aβ25-35 or 5 μM Aβ1-42 (Veh). Three days later, the Aβ was removed and one of the compounds (0.01, 0.1, 1, and 10 μM), NGF (100 ng/ml), or vehicle (0.1% DMSO) was added to the neurons. Four days after the treatment, the neurons were fixed and immunostained for pNF-H and MAP2. The lengths of the pNF-H positive neurites were measured. (A) Representative images of pNF-H-positive axons and MAP2-positive neurons in Aβ25-35 treated group. Scale bar, 200 μm. (B) Time course of the experiments (Upper). Quantification of total axonal outgrowth and associated statistics (Lower). The error bars represent SEM. One-way ANOVA and Dunnett’s post hoc test were performed (∗p < 0.05 vs. Aβ/Veh; n = 10–40 photographs).