FIGURE 2.

Advanced oxidative protein products -induced DRG neurons apoptosis. (A) Viability of DRG neurons treated with AOPPs at different concentrations. (B) Representative dot blots of Annexin V–FITC versus PI. Primary DRG neurons were incubated with AOPPs–RSA for the indicated concentrations or unmodified RSA for 24 h. (C) DRG neurons were incubated with 100 μg/mL AOPPs–RSA for the indicated time durations. Apoptosis was quantified by measuring the number of combined early and late apoptotic cells using flow cytometry and was found to increase in a concentration and time-dependent manner. Data represent mean ± SEM of at least three independent experiments. ^∗P < 0.05 versus control group (or time = 0).