Fig. S1.

Infusion of Ad-Munc18b into the pancreas of normal Wistar rats improves glucose homeostasis. (a) Islets from Wistar rats were isolated 2 h after pancreatic ductal infusion with Ad-Munc18b/eGFP, and placed into culture. Top: 3D reconstruction of 0.3 μm-thick optical confocal sections of islets at 1 and 2 days in culture. Bottom: corresponding images at different sections of the islet at 2 day culture. Scale bar: 25 μm. (b) Confocal images of islets isolated from Wistar rats at 2 weeks and 4 weeks post ductal infusion of Ad-Munc18b/eGFP (right; phase contrast images on left). Munc18b/eGFP expression remained optimal at 2 weeks (top, similar to the 2 day culture) but was substantially reduced at 4 weeks (bottom). Scale bar: 50 μm. (c) IPGTTs performed on normal Wistar rats treated with Ad-Munc18b/eGFP at 1-week, 2-week and 4-week post-op. Blood glucose (left) and insulin levels (right) are shown along with the AUCs encompassing 120 min of the IPGTT. N = 4 for each group. Data are shown as mean ± SEM; *p < 0.05, **p < 0.01, NS: no significant difference.