Fig. 4.

p53 and p53-dependent genes. (a) Quantity of p53 protein by automated Wes Western blotting in WT-CPCs (WT) and p53-tg-CPCs (p53-tg) at baseline (blue line) and after Doxo (red line). Tracings illustrate the peak level of p53 in the four CPC classes; n = 3 in all cases. (b) The pseudo-blots show the expression of phosphorylated p53 at Ser-18 and Ser-34, and p53 and GAPDH in the four CPC classes. (c) Quantitative data are shown as mean ± SD. *p < 0.05 vs. WT Ctrl. **p < 0.05 vs. WT Doxo. ***p < 0.05 vs. p53-tg Ctrl. (d) mRNA level of p53 and p53 regulated genes in the CPC classes at baseline (Ctrl) and after Doxo; n = 3 in all cases. Ct values above 35 cycles were considered not detectable. For statistics see panel B.