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. 2017 Jun 19;8:1059. doi: 10.3389/fpls.2017.01059

Figure 3.

Figure 3

MiR156 affects adventitious rooting capacity in transgenic tobacco plants. (A) Phenotypes of 13-day-old plants (wild-type, 35S:MdMIR156a6, and 35S:MIM156) grown on hormone-free MS medium. Scale bars = 20 mm. (B) Relative expression level of miR156 in transgenic tobacco lines. Bars show SD from three biological replicates. (C) Relative expression levels of NbSPLs in WT, 35S:MdMIR156a6, and 35S:MIM156 plants in stems. (D,E) Rooting ability of tobacco stem cuttings. (D) Adventitious root number per cutting and (E) percent rooting. Adventitious root number was counted after 13 days of growth on MS medium. Bars show SD from three biological replicates; n = 10 in individuals per replicate. Asterisks indicate significant differences from the WT (Student's t-test, **P < 0.01, *P < 0.05).