Table 2.
Kinetic parameters for substrates of the E. coli ADP-GlcPPase in the pyrophosphorolysis direction.
| CONTROL | + Fru-1,6-bisP | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Pyrophosphorolysis of | Substrate | S0.5 (mM) | n | kcat (s−1) | kcat/S0.5(s−1 mM−1) | S0.5 (mM) | n | kcat (s−1) | kcat/S0.5(s−1 mM−1) |
| ADP-Glc | ADP-Glc | 0.42 ± 0.04 | 1.3 ± 0.1 | 12 ± 2 | 27 | 0.20 ± 0.02 | 2.1 ± 0.2 | 110 ± 6 | 550 |
| PPi | 0.50 ± 0.04 | 2.7 ± 0.3 | 23 | 0.07 ± 0.01 | 1.0 ± 0.1 | 1572 | |||
| Mg2+ | 5.4 ± 0.5 | 1.8 ± 0.2 | 2 | 2.2 ± 0.2 | 2.0 ± 0.2 | 50 | |||
| UDP-Glc | UDP-Glc | 2.4 ± 0.3 | 1.0 ± 0.1 | 0.41 ± 0.05 | 0.1 | 2.2 ± 0.2 | 0.9 ± 0.1 | 0.40 ± 0.03 | 0.1 |
| PPi | 0.54 ± 0.04 | 1.8 ± 0.1 | 0.8 | 0.50 ± 0.05 | 1.8 ± 0.1 | 0.8 | |||
| Mg2+ | 5.5 ± 0.5 | 1.7 ± 0.1 | 0.1 | 5.2 ± 0.2 | 2.1 ± 0.2 | 0.1 | |||
| GDP-Glc | GDP-Glc | 4.9 ± 0.4 | 2.3 ± 0.3 | >0.33 | 0.1 | 4.9 ± 0.3 | 1.7 ± 0.2 | >0.30 | 0.1 |
| PPi | >1 | N/A | N/A | >1 | N/A | N/A | |||
| Mg2+ | 4.4 ± 0.5 | 1.9 ± 0.2 | 0.1 | 4.7 ± 0.3 | 2.6 ± 0.4 | 0.1 | |||