Table 3.
Kinetic parameters for ITP.
CONTROL | + Fru-1,6-bisP | |
---|---|---|
S0.5(mM) | 0.55 ± 0.04 | 0.50 ± 0.03 |
n | 2.6 ± 0.4 | 1.8 ± 0.2 |
kcat (s−1) | 0.55 ± 0.01 | 0.90 ± 0.01 |
kcat/S0.5 (s−1 mM−1) | 1 | 1.8 |
(kcat/S0.5)ATP/(kcat/S0.5)*ITP | 1 | 99 |
Data for ATP come from Table 1. Concentrations of Glc-1P (1.5 mM) and Mg2+ (10 mM) were saturating and assays were done as described in Materials and Methods.