. 2017 Apr 20;7(6):e00708. doi: 10.1002/brb3.708

Table 1.

Locomotor activity and bout analysis. Male mice exhibited decreased locomotor activity compared to females on RC. Additionally, males were also more sensitive to the negative effects of HFD exposure as they exhibited reduced activity over time compared to females. Alcohol produced no alterations to locomotor activity in either sex. Italics indicate significant differences between Before and After activity parameters, and different letters indicate significantly different from each other, at p <.05

Group	N	Sex	Diet	Fluid	Average	Light average	Dark average	LD Ratio	Bout length (min)	Counts per bout	Bouts per day
Before weeks 10–12
Controls	5	M	RC	H₂O	19.2 ± 1.7^a	8.5 ± 0.6^a	29.9 ± 3.1^a	0.28 ± 0.09	47.8 ± 12.5^a	226.3 ± 33^a	11.1 ± 0.5^a
Controls	4	F	RC	H₂O	44.5 ± 6.3^b	17.2 ± 2.0^b	71.8 ± 14.4^b	0.29 ± 0.02	73.6 ± 3.9^b	779.9 ± 220.0^b	8.1 ± 1.1^b
Diet Pref.	6	M	HFD/RC	H₂O	13.8 ± 1.1 ^a *	6.4 ± 0.8^a*	21.2 ± 2.7 ^a *	0.31 ± 0.02	48.2 ± 5.0 ^a	226.3 ± 33.7 ^a	8.1 ± 0.4*
	5	M	HFD/RC	10% EtOH	14.4 ± 1.1 ^a *	5.38 ± 0.5^a*	23.46 ± 2.1 ^a	0.23 ± 0.03	48.4 ± 2.4 ^a	237.6 ± 18.7 ^a	7.9 ± 0.4*
	4	F	HFD/RC	H₂O	44.0 ± 7.0^b	17.1 ± 3.0^b	70.9 ± 15.5^b	0.26 ± 0.07	76.0 ± 15.5^b	787.5 ± 271.4^b	7.8 ± 1.2
	4	F	HFD/RC	10% EtOH	39.3 ± 6.8^b	14.5 ± 2.5^b	64.2 ± 11.9^b	0.23 ± 0.04	75.7 ± 9.5^b	713.4 ± 168.2^b	7.2 ± 0.3
Fluid Pref.	6	M	TEN	H₂O/10% EtOH	20.1 ± 2.6^a	8.0 ± 1.1^a	32.1 ± 4.3^a	0.25 ± 0.02	39.4 ± 2.8^a*	231.3 ± 34.5^a	11.4 ± 0.3^a
	6	M	HFD	H₂O/10% EtOH	20.9 ± 1.5^a	8.5 ± 1.0^a	33.3 ± 2.1 ^a	0.26 ± 0.02	36.3 ± 1.0^a*	214.2 ± 14.7^a	12.8 ± 0.4^a
	4	F	TEN	H₂O/10% EtOH	53.5 ± 12.3^b	21.7 ± 9.7^b	85.3 ± 15.1^b	0.24 ± 0.06	73.6 ± 10.2^b	847.1 ± 215.4^b	8.1 ± 0.2^b
	4	F	HFD	H₂O/10% EtOH	48.5 ± 6.4^b	15.9 ± 3.5^b	81.2 ± 11.7^b	0.20 ± 0.10	77.9 ± 15.3^b	868.2 ± 181.9^b	7.4 ± 0.8^b
After weeks 13–15
Controls	5	M	RC	H₂O	17.6 ± 1.9^a	7.7 ± 0.5^a	27.6 ± 4.0^a	0.29 ± 0.03	44.6 ± 3.8^a	214.2 ± 31.1^a	10.7 ± 0.2^a
Controls	4	F	RC	H₂O	38.1 ± 6.5^b	14.8 ± 1.2^b	61.4 ± 14.3^b	0.30 ± 0.10	61.1 ± 9.4^b	580.0 ± 166.9^b	8.9 ± 1.2^b
Diet Pref.	6	M	HFD/RC	H₂O	11.9 ± 1.2 ^a *	5.3 ± 0.6^a*	18.5 ± 1.9 ^a *	0.29 ± 0.02	41.1 ± 2.6 ^a	169.1 ± 18.6 ^a	9.1 ± 0.4^a*
	5	M	HFD/RC	10% EtOH	12.1 ± 0.4 ^a *	5.0 ± 0.4^a*	19.2 ± 0.6 ^a *	0.26 ± 0.02	41.6 ± 1.5 ^a	181.4 ± 9.9 ^a	8.6 ± 0.5^a*
	4	F	HFD/RC	H₂O	37.0 ± 9.5^b	15.2 ± 2.4^b	58.7 ± 19.9^b	0.30 ± 0.10	78.7 ± 23.9^b	730.3 ± 353.7^b	7.3 ± 1.3^b
	4	F	HFD/RC	10% EtOH	35.3 ± 6.6^b	12.2 ± 1.8^b	58.3 ± 11.7^b	0.21 ± 0.03	69.6 ± 7.3^b	610.1 ± 150.9^b	7.6 ± 0.2^b
Fluid Pref.	6	M	TEN	H₂O/10% EtOH	19.2 ± 2.5a	10.0 ± 1.4^a	28.3 ± 3.7^a	0.36 ± 0.02	34.4 ± 2.6^a*	193.7 ± 32.0^a	13.1 ± 0.5^a*
	6	M	HFD	H₂O/10% EtOH	18.0 ± 0.9a	8.9 ± 0.3^a	27.1 ± 1.4 ^a	0.33 ± 0.01	34.6 ± 1.2^a*	185.4 ± 11.5^a	12.7 ± 0.4^a*
	4	F	TEN	H₂O/10% EtOH	52.5 ± 9.7b	19.1 ± 7.2^b	85.8 ± 12.1^b	0.21 ± 0.04	75.2 ± 5.7^b	859.9 ± 152.9^b	7.7 ± 0.5^b
	4	F	HFD	H₂O/10% EtOH	46.6 ± 4.8b	14.2 ± 2.0^b	79.0 ± 8.4^b	0.18 ± 0.02	95.4 ± 19.7^b	1046.0 ± 291.5^b	6.2 ± 0.9^b