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. 2017 Jun 13;8:15803. doi: 10.1038/ncomms15803

Figure 5. Effects of mitotic delay on daughter centriole maturation.

Figure 5

(a,b) RPE1 cells expressing eGFP centrin-2 and probed for CEP164 in unsynchronized, G2-synchronized and mitotically arrested cells. Lower left bar, 10 μm. Lower right bar, 1 μm. (b) Quantification of multiple CEP164 foci from experiments illustrated in a. Error bars represent s.e.m. for three replicate experiments, 300 cells scored per condition. (c) Quantification of CEP164 foci in cells transfected with control or separase siRNA followed by prometaphase arrest. Error bars represent s.e.m. for three replicate experiments, 300 cells scored per condition per experiment. (d) Quantification of CEP164 foci in cells subjected to APC/C inhibition during prometaphase arrest. Error bars represent s.e.m. for six replicate experiments, 300 cells scored per condition per experiment. For b–d, significance was determined by one-way ANOVA with Tukey–Kramer post hoc test, ****P≤0.0001.