Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jun 13;8:15691. doi: 10.1038/ncomms15691

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017, The Author(s)

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(a) Twenty-four-month-old C57Bl/6^(INK-ATTAC) male mice were split into three groups and assigned to control (n=6), AP20187 (n=6) or senolytic (D+Q, n=6) treatment for 3 months. Percentage (b) of hepatocytes positive for TAF, (c) of karyomegalic hepatocytes and (d) of Oil Red O staining per area was decreased in animals treated with AP20187 and senolytic drugs at 27 months of age. (e) Six-month-old C57Bl/6^(INK-ATTAC) male mice were split into four groups and assigned to chow (n=8) or high fat (HF, n=8) diet and treated at 11 months of age with vehicle (n=8) or AP20187(n=8) treatment until 15 months of age. (f) Percentage of hepatocytes positive for ≥3 TAF (n=6) and (g) percentage of karyomegalic hepatocytes area were significantly increased in animals fed a HF diet and significantly decreased in animals treated with AP20187 (n=6–7). RNA-ISH for (h,i) p16 and (j,k) eGFP shows a significant increase in p16- and eGFP-positive hepatocytes in HF-fed mice and a significant decrease after treatment with AP20187. (i,k) Representative images of IHC RNA-ISH staining for eGFP and p16 in mouse liver (red/pink=p16/eGFP, blue=haematoxylin, n=6–8, 10–20 images per liver). Scale bars, 100 μm. (l) Percentage of Oil Red O staining per area increases significantly in mice on HF diet and decreases after treatment with AP20187 (n=6–7). All data are mean±s.d. with 6–8 animals per group. Significant differences (one-way analysis of variance) are indicated with *P≤0.05 and **P≤0.001.