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. 2017 Jun 13;8:15691. doi: 10.1038/ncomms15691

Figure 5. Induction of senescence in hepatocytes causes mitochondrial dysfunction and reduces fatty acid oxidation capacity.

Figure 5

Hepatocytes isolated from wild-type C57Bl/6 mice acquire a senescent phenotype 6 days after 10 Gy X-ray irradiation: (a) representative images showing SA-β-Gal in non-irradiated cells (NOIR) and 6 days after irradiation, Merge: light blue=DAPI, dark blue=SA-β-Gal, scale bar, 20 μm; (b) frequencies of SA-β-Gal-positive hepatocytes and (c) representative images showing 53BP1 staining in NOIR and 6 days after irradiation. Merge: blue=DAPI, red=53BP1, scale bar 10 μm; (d) average number of 53BP1 foci per cell are significantly increased 6 days after irradiation. (e) Representative images showing Nile red staining in NOIR and 6 days after IR. Merge: blue=DAPI, red=Nile red, scale bar scale bar 20 μm. (f) Average fluorescence intensity of Nile red staining in senescent or non-senescent hepatocytes. (g) Change in OCR in senescent or non-senescent hepatocytes after addition of the fatty acid palmitate as substrate; (h) change in OCR in senescent or non-senescent hepatocytes after inhibition of fatty acid oxidation by etomoxir. Data in b,g,h are mean±s.e.m. and in d,f are mean±s.d. with 3–4 animals per group. Significant differences (t-test) are indicated with *P≤0.05 and **P≤0.001.