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. 2017 Jun 13;8:15832. doi: 10.1038/ncomms15832

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Copyright © 2017, The Author(s)

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Top: CHX chase analysed by anti-Fzo1 and anti-Pgk1 immunoblotting with WT and mga2Δ cells (DF5 background). MW in kDa are shown on the right of indicated regions of immunoblots. Bottom: quantification of CHX chases. Error bars represent the s.e.m. from three independent experiments. (b) Dextrose and glycerol spot assays with WT, ubp2Δ, mga2Δ and ubp2Δ mga2Δ (clone 3) cells (DF5 background) transformed with an empty plasmid (Vector) or pRS314-FZO1 (FZO1). (c) Percentage of cells with tubular (blue) or fragmented/aggregated (green) mitochondria from strains used in b but transformed with pRS314-FZO1. Error bars represent the s.d. from three independent experiments. **P<0.05, ***P<0.005 (one-way analysis of variance (ANOVA)). NS, not significant. More than 95 cells were analysed per sample. (d) Total protein extracts prepared from strains in b were analysed by anti-Fzo1 and anti-Pgk1 immunoblotting. MW in kDa are shown on the right of indicated regions of immunoblots. (e) Dextrose and glycerol spot assays with ubp2Δ mga2Δ (clone 3) cells (DF5 background) transformed with an empty plasmid (Vector), pRS314-FZO1 (FZO1) or pRS314-FZO1 K398R (K398R).