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. Author manuscript; available in PMC: 2017 Jun 19.
Published in final edited form as: J Appl Toxicol. 2013 May 20;34(5):480–488. doi: 10.1002/jat.2888

Figure 2.

Figure 2

Effect of ketamine on E2 levels in zebrafish embryos. Embryos at 48 h post fertilization (hpf) were exposed to 2 mM ketamine for 24 h (static exposure). Post exposure, embryos were washed extensively with quick changes of embryo water three times. Reverse-phase high-performance liquid chromatography (HPLC) was employed to measure estradiol-17β (E2) levels in the embryos (n = 50 per group). Data from three separate experiments were averaged and are presented as mean ± SD. Student’s t test was used to determine significance P < 0.05 (*).