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. Author manuscript; available in PMC: 2017 Jun 19.
Published in final edited form as: J Appl Toxicol. 2013 May 20;34(5):480–488. doi: 10.1002/jat.2888

Figure 5.

Figure 5

Effect of ketamine and estradiol-17β (E2) on activated ERK (phosphorylated ERK or p-ERK) levels in zebrafish embryos. Ketamine (2 mM) or E2 (1 nM) treatment of 48 h post fertilization (hpf) embryos was for 24 h (static exposure). (A) Western blot analysis conducted on whole-embryo lysates (one embryo/lane) shows activated ERK (p-ERK) and total ERK levels (on the same blot used after stripping). (B) Densitometric analysis (presented as the ratio of the densities of p-ERK to total ERK) of the Western blots. Data from three separate experiments were averaged and shown as the mean ±SD. Student’s t test was used to compare the densitometric values of the P-ERK/Total ERK of the control and the respective treated groups. *Significance was set at P < 0.05.