Table S3.

SUIT protocol 1

No.	SUIT	State	Figs.
1	Malate, 5 mM		Fig. 4D and Fig. S2
	Octanoyl carnitine, 0.2 mM	OctML
2	ADP, 10 mM*	OctMP	Fig. 2D and Fig. S2
3	Glutamate, 10 mM	OctGMP	Fig. S2
4	Succinate, 10 mM	OctGMSP	Fig. S2
5	Cytochrome c, 10 μM	OctGMScP
6	FCCP, 0.25–1.5 μM†	OctGMSE	Fig. S2
7	Rotenone, 0.5 μM	SE	Fig. S2

Reagents were added in the order 1–7 (stub column) to give the final concentrations shown. c, cytochrome c; _L, LEAK state respiration; _P, OXPHOS state respiration; S_E, S-ETS capacity.

^*The minimum amount of ADP added was 10 mM. Higher concentrations were required to reach saturation in some cases.

^†FCCP was titrated in 0.25 μM steps until an inhibitory effect was observed.