Table S4.

SUIT protocol 2

No.	SUIT	State	Figs.
1	Malate, 5 mM
	Glutamate, 10 mM	GML	Fig. S3
2	ADP, 10 mM*	GMP	Fig. 4A and Fig. S3
3	Cytochrome c, 10 μM	GMcP
4	Succinate, 10 mM	GMSP	Fig. 4C and Fig. S3
5	FCCP, 0.25–1.5 μM†	GMSE	Fig. S3
6	Rotenone, 0.5 μM	SE	Fig. 4B and Fig. S3

Reagents were added in the order 1–6 (stub column) to give the final concentrations shown.

^*The minimum amount of ADP added was 10 mM. Higher concentrations were required to reach saturation in some cases.

^†FCCP was titrated in 0.25 μM steps until an inhibitory effect was observed.