Fig. 4.

Contribution of initiation complex components on mRNA recruitment. eIF3j-Fl vs. 48S PIC saturation curves (Left) and the calculated equilibrium dissociation constants (Right) as described in Fig. 1B using CAA(AUG)-42 mRNA. Shown is eIF3j-Fl affinity in the presence or absence of 4GΔN, m⁷G cap, eIF4E, and eIF4B (A) or of eIF1, eIF1A, and eIF5 (B). Note that the concentrations of eIF4A, eIF4E, and eIF4B were increased twofold in the absence of eIF4G, to compensate for a possible decrease in affinity of these components. Green dashed lines and green shaded areas represent the control data obtained with the complete set of 48S PIC components and CAA(AUG)-42 mRNA (Fig. 1C). Data represent mean ± SD of three independent experiments.