Fig. 4.

ICP27-PABP–mediated translational stimulation is dependent on eIF4G. (A) Y2H of PABP RRM1-2 (amino acids 1–182) or RRM1-2Nt (amino acids 3–137) with ICP27 (amino acids 10–512), eIF4G (amino acids 1–641), or Paip1. MS2 and IRP were used negative controls. (B) Immobilized purified GST-ICP27 or GST was incubated with purified FLAG-eIF4G and/or His-PABP as indicated. Proteins were detected by immunoblotting. (C) Oocytes expressing MS2-U1A (negative control), MS2-ICP27, MS2-PABP, or MS2-SLBP were injected with m⁷G-Luc-MS2₃ and CSFV–β-gal mRNAs as well as mRNAs expressing either 2Apro or U1A (n = 4; ±SEM). *P < 0.05; not significant (n.s.), P > 0.05.