Table S1.
Y2H analysis of the interaction of ICP27 and translation initiation factors
| Binding domain | Activation domain | Interaction* |
| Lex A | ICP27 | − |
| eIF4G Ct | ICP27 | − |
| PABP Ct | ICP27 | ++++ |
| ICP27 | IRP | − |
| ICP27 | PABP Ct | +++ |
| ICP27 | eIF1 | − |
| ICP27 | eIF1A | − |
| ICP27 | eIF2α | +/− |
| ICP27 | eIF2β | +/− |
| ICP27 | eIF3 S1 | − |
| ICP27 | eIF3 S2 | +/− |
| ICP27 | eIF3 S3 | − |
| ICP27 | eIF3 S4 | + |
| ICP27 | eIF3 S5 | +/− |
| ICP27 | eIF3 S6 | +/− |
| ICP27 | eIF3 S7 | +/− |
| ICP27 | eIF3 S8 | − |
| ICP27 | eIF4A | +/− |
| ICP27 | eIF4B | − |
| ICP27 | eIF4E | +/− |
| ICP27 | eIF4G Nt | − |
| ICP27 | eIF4H | − |
| ICP27 | eIF5 | − |
| ICP27 | eIF5A | − |
| ICP27 | eIF5B | − |
| ICP27 | Paip 1 | − |
Ct, C terminus; Nt, N terminus.
*
Y2H interactions were determined with a β-gal filter assay as described (24). Color development is denoted by a + symbol, with multiple + symbols representing increasing intensity relative to the negative control. The symbol +/− represents faint color development in some colonies.