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Amino acid sequence alignments of known AcsF proteins. Sequences are those from Rvi. gelatinosus (AcsF), Synechocystis (CycI), C. reinhardtii (CRD1), A. thaliana (CHL27), and Rba. sphaeroides (Rsp_0294; abbreviated as 0294). Conserved, highly similar, and similar residues are highlighted in black, dark gray, and light gray, respectively. The putative diiron center ligands are marked by red diamonds.
