Fig. S2.

Genetic knockouts and replacements in Rvi. gelatinosus. (A) Depiction of the deletion of bchE (Left), confirmed by colony PCR (Right). (B) Depiction of deletion of acsF, and subsequent integration of foreign genes at the acsF locus, under control of the native promoter (Upper), confirmed by colony PCR (Lower). The regions subjected to genetic manipulation are depicted in proportion to the scale bar. ORFs are represented as colored filled rectangles, within which the arrow indicates the direction of transcription. Crt, carotenoid biosynthesis; RC&LHC, reaction center and light-harvesting complexes.