Table S2.
Strains and plasmids described in this study
| Strain/plasmid | Genotype/characteristics | Source |
| E. coli | ||
| JM109 | Cloning strain for plasmid constructs | Promega |
| S17-1 | Conjugation strain for pK18mobsacB constructs | (48) |
| Rvi. gelatinosus | ||
| WT | IL144 | S. Nagashima* |
| ∆bchE | Unmarked deletion mutant of bchE in WT | This study |
| ∆bchE∆acsF | Unmarked deletion mutant of acsF in ∆bchE | This study |
| ∆bchE∆acsF::acsFRs | acsFRs replacement of acsF in ∆bchE | This study |
| ∆bchE∆acsF::bciE-acsFRs | acsF replaced with rsp_6110-acsFRs in ∆bchE | This study |
| ∆bchE∆acsF::cycI | cycI replacement of acsF in ∆bchE | This study |
| ∆bchE∆acsF::cycI-ycf54 | cycI-ycf54 replacement of acsF in ∆bchE | This study |
| Synechocystis | ||
| WT | sp. PCC6803 | R. Sobotka† |
| acsFRg+ | acsFRg and KmR replacement of psbAII in WT | This study |
| acsFRg+ ∆cycI | CmR replacement of cycI in acsFRg+ | This study |
| acsFRg+ ∆cycI∆ycf54 | ZeoR replacement of central portion of ycf54 in acsFRg+∆cycI | This study |
| ∆ycf54 | ZeoR replacement of central portion of ycf54 in WT | (22) |
| Rba. sphaeroides | ||
| WT | 2.4.1 | S. Kaplan‡ |
| ∆bchE∆ccoP | Unmarked deletion mutant of bchE and ccoP in WT | (15) |
| ∆bchE∆ccoP∆acsF | Unmarked deletion mutant of acsF in ∆bchE∆ccoP | (15) |
| ∆bchE∆ccoP∆6110 | Unmarked deletion mutant of rsp_6110 in ∆bchE∆ccoP | This study |
| Plasmids | ||
| pK18mobsacB | Allelic exchange vector, KmR | J. Armitage§ |
| pK18∆bchERg | Upstream-NdeI-downstream of bchERg cloned into BamHI/HindIII sites of pK18mobsacB | This study |
| pK18∆acsFRg | Upstream-NdeI-downstream of acsFRg cloned into BamHI/HindIII sites of pK18mobsacB | This study |
| pK18∆6110 | Upstream-downstream of rsp_6110 cloned into XbaI/HindIII sites of pK18mobsacB | This study |
| pK18[acsFRs] | acsFRs cloned into the NdeI site of pK18∆acsFRg | This study |
| pK18[6110-acsFRs] | rsp_6110-acsFRs cloned into the NdeI site of pK18∆acsFRg | This study |
| pK18[cycI] | cycI cloned into the NdeI site of pK18∆acsFRg | This study |
| pK18[cycI-ycf54] | cycI-ycf54 cloned into the NdeI site of pK18∆acsFRg | This study |
| pPD-FLAG | Cloning site, KmR, flanked by psbAII upstream and downstream regions, AmpR | (21) |
| pPD[acsFRg] | acsFRg cloned into NdeI/BglII sites of pPD-FLAG | This study |
| pBBRBB-Ppuf843–1200 | Expression vector carrying the 843–1,200 region of puf promoter of Rba. sphaeroides, KmR | (27) |
| pBB[6110] | rsp_6110 cloned into the BglII/NotI sites of pBBRBB-Ppuf843–1200 | This study |
*
Research Institute for Photosynthetic Hydrogen Production, Kanagawa University, Yokohama, Japan.
†
Institute of Microbiology, Department of Phototrophic Microorganisms, Třeboň, Czech Republic.
‡
Department of Microbiology and Molecular Genetics, University of Texas Medical School, Austin, TX.
§
Department of Biochemistry, University of Oxford, Oxford, United Kingdom.