Fig. S3.

Mlph’s phosphorylation state does not interfere substantially with actin binding. Dephosphorylated Alexa Fluor 647-labeled Rab27a/Mlph complex (cyan) was mixed in equal amounts with phosphorylated, Alexa 488-labeled Rab27a/Mlph complex (green) and was incubated with surface-attached, Atto565-labeled actin filaments (red; dye swap control for Fig. 2B). The quantification showed results identical to those demonstrated in Fig. 2B: Phosphorylation of Mlph does not alter the interaction between Mlph and actin filaments. Error bars represent SD. (Scale bar: 3 µm.)