Fig. 4.
Targeting of tonoplast proteins but not FERONIA the key plasma membrane receptor is interfered in synergids by AP1G loss of function. (A and B) CLSM of ProDD39:GFP-NRT2.7 in synergids of WT or ap1g1/+ g2. Pistils from ProDD39:GFP-NRT2.7 or ProDD39:GFP-NRT2.7;ap1g1/+ g2 plants were either emasculated at maturation for visualization (A) or emasculated and pollinated with ProLAT52:DsRed pollen and visualized at 8 HAP (B). Merges of GFP, RFP, and BF channels are shown at the right side in B. Dotted lines illustrate synergid cells. (C–F) CLSM of ProDD39:FER-YFP in synergids of WT (C and D) or ap1g1/+ g2 (E and F). Pistils from ProDD39:FER-YFP or ProDD39:FER-YFP;ap1g1/+ g2 plants were either emasculated at maturation for visualization (C and E) or emasculated and pollinated with ProLAT52:DsRed pollen and visualized at 8 HAP (D and F). Merges of GFP, RFP, and BFA channels are shown at the right side in D and F. Insets in C and E are their corresponding close-ups at the micropylar region. dPt, discharged pollen tube; Pt, pollen tube. (Scale bars: A and B, 10 µm; D and F and C and E, Inset, 25 µm.)