Fig. S5.

AP1G-mediated and FER-mediated pathways are genetically independent. (A) A representative fer-4/+ ap1g1/+ g2/g2 pistil emasculated and hand-pollinated with WT pollen and examined by aniline blue staining at 48 HAP. On the top right is the ratio of undeveloped ovules. Four overlapping high-magnification images were taken for one pistil. The images were then overlaid with Photoshop (Adobe Systems) to show the whole pistil. Results shown are means ± SD (n = 15 pistils) (B–D) Close-up of rectangle boxes showing in A from left to right, showing an developed ovule (B) and two representative ovules with tangled pollen tubes inside (C and D). Arrowheads point at the micropyle. (E) A representative fer-4/+ ap1g1/+ g2/g2 silique. Arrowheads point at undeveloped seeds. (Scale bars: A, 200 µm; B–D, 50 µm; E, 500 µm.)