Fig. S8.
VPE-mediated vacuolar rupture does not contribute to pollen tube reception. (A) Representative histochemical GUS analysis of the quadruple VPE mutant (atvpe) pistils emasculated and hand-pollinated with ProLAT52:GUS pollen at 9 HAP. (B) Aniline blue staining of atvpe pistil emasculated and hand-pollinated with WT pollen at 48 HAP. Two overlapping high-magnification images were taken for one pistil. The images were then overlaid with Photoshop (Adobe Systems) to show the whole pistil. (C) A representative silique in self-fertilized atvpe. (D–F) Quantitative analysis of pollen tube guidance from ProLAT52:GUS pollination assay (D), pollen tube reception from aniline blue staining assay (E), or seed sets from silique analysis (F). Results are means ± SEM of six replicate experiments. (Scale bars: A and B, 200 µm; C, 1 mm.)