Fig. 5.

Otu genetically interacts with bam. (A–C) Ovaries from P{hs-bam} (hs-) (A, wild-type control), P{hs-bam} (hs+) (B), and otu¹;P{hs-bam} (hs+) (C) flies were stained with anti-Vasa (green) and anti-Hts (red) antibodies. (D) Ovaries from P{nosp-gal4:vp16} (wild-type control) and P{nosp-gal4:vp16};P{uasp-art-miRotu} were dissected and subjected to quantitative RT-PCR (qRT-PCR) assays. Error bars represent SD (n = 3). (E) The staining was performed as in C, except that flies used were P{uasp-art-miRotu}/P{hs-bam,hs-gal4} (hs+). (Scale bars: 10 μm.) (F) Quantification of the germarium phenotypes of ovaries in A–C and E. In D, the Student’s t test was used to analyze statistical variance. **P < 0.01.