Table S1.

Identification of the Dub that regulates CycA deubiquitination by a cell-based screening

Group	CG numbers of potential Dub genes
A	CG1490, CG3251, CG4265, CG5794, CG7857, CG8877, CG14884
B	CG1945, CG3416, CG4751, CG5798, CG8232, CG9448, CG15817
C	CG1950, CG3431, CG4968, CG6091, CG8334, CG9769, CG18174
D	CG2224, CG3781, CG5384, CG6932, CG8445, CG12082, CG30421
E	CG2904, CG4165, CG5486, CG7023, CG8494, CG12743, CG32479
F	CG3016, CG4166, CG5505, CG7288, CG8830, CG14619

To identify the specific Dub(s) that regulates CycA deubiquitination, we used indicated dsRNAs in Table S1 to treat S2 cells and performed cell-based deubiquitination assays. In this experiment, 41 candidate genes encoding potential Dubs were divided into 6 groups and indicated dsRNAs of each group were mixed to treat S2 cells for 48 h, the treated cells were then transfected with HA-Ub and Flag-CycA plasmids. Cell lysates were used to perform in vivo deubiquitination assays.