Table 1.
Tobacco Genome Assembly Statistics
| Nitab4.5 NGS assembly |
S sub-genome | T sub-genome | BioNano Optical Map | Hybrid assembly | Pseudo-molecules | |
|---|---|---|---|---|---|---|
| Number of contigs/scaffolds | 1,084,432 | 386,863 | 228,210 | 3945 | 2217 | 24 |
| Total length (Mb) | 4695 | 2418 | 1859 | 3932 | 3688 | 2924 |
| Total defined bases (Mb) | 4049 | 1997 | 1544 | – | – | 1742 |
| Max length (Mb) | 5.99 | 2.21 | 5.99 | 9.04 | 13.67 | 216 |
| N50 length (Mb) | 0.28 | 0.21 | 0.66 | 1.33 | 2.17 | 116 |
| Anchored sequence (Mb) | 2924 | 715 | 1124 |
Table showing assembly statistics for the different levels of the tobacco genome assembly. Statistics for the S and T sub-genomes are based on calling ancestral origin of Nitab4.5 NGS assembly following mapping of sequence reads from N. sylvestris and N. tomentosiformis [17]