FIGURE 5.

atgrxs17 seedlings increase ROS production under iron deficiency stress. Wild type control, atgrxs17 KO, and AtGRXS17 RNAi seeds were germinated and vertically grown on ½ MS medium for 5 days, then transferred to and grown on iron sufficient or deficient medium for 3 days. Seedlings were stained with DHE to monitor ROS levels. Shown are representative images for iron sufficient (A) and deficient (B) treatments. Scale Bars = 50 μm. (C) The intensity of ROS signals were captured by confocal microscope and analyzed with Image J. Student’s t-test, n = 6–10, ^∗∗p < 0.01.