Skip to main content
. Author manuscript; available in PMC: 2018 Apr 11.
Published in final edited form as: Biochemistry. 2017 Mar 31;56(14):2010–2023. doi: 10.1021/acs.biochem.7b00137

Figure 3. Catalytic activity measurements.

Figure 3

(a) The cyclization of GGPP by full-length TbTS exhibits Michaelis-Menten kinetics with kcat = 0.16 s−1, KM = 2.9 μM, and kcat/KM = 5.5 × 104 M−1s−1. The highest concentrations of GGPP are not included in the final curve due to substrate inhibition. (b) The cyclization of GGPP by TbTSαβG exhibits Michaelis-Menten kinetics with kcat = 0.000019 s−1, KM = 0.96 μM, and kcat/KM = 20 M−1s−1.