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. Author manuscript; available in PMC: 2018 Apr 11.
Published in final edited form as: Biochemistry. 2017 Mar 31;56(14):2010–2023. doi: 10.1021/acs.biochem.7b00137

Table 3.

Steady-state kinetics for wild-type and chimeric bifunctional αα terpene synthases with substrate GGPP

Enzyme PaFSa AcOS AcOSα-PaFSα AcOSα′-PaFSα
kcat (s−1) 0.021 ± 0.002 0.0069 ± 0.001 0.011 ± 0.002 0.0012 ± 0.0003
KM (μM) 0.63 ± 0.03 0.024 ± 0.01 0.038 ± 0.01 0.48 ± 0.2
h 2.2 1 1 1.8
kcat/KM (M−1s−1) 3.3 × 104 2.9 × 105 2.9 × 105 2.5 × 103
a

From ref. 30.