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. Author manuscript; available in PMC: 2018 May 3.
Published in final edited form as: Neuron. 2017 Apr 13;94(3):581–594.e5. doi: 10.1016/j.neuron.2017.03.043

Figure 5. Mfsd2a−/− mice display an increased number of Cav-1-positive vesicles in CNS endothelial cells. See also Table S4.

Figure 5

(A–H) Cav-1 immunoreactivity in CNS endothelial cells of Mfsd2a+/+ and Mfsd2a−/− mice under electron microscopy. Electron microscopy analysis with immuno-gold labeling of Cav-1 protein in CNS endothelial cells of Mfsd2a+/+ (A–D) and Mfsd2a−/− mice (E–H) at P90. Gold particles (black puncta) are visualized along the luminal (arrows) and abluminal (Ab; black arrowheads) plasma membranes, as well as within the cytoplasm. Cytoplasmic particles are seen associated with vesicular structures (white arrowheads). All scale bars, 500 nm. (I) Quantification of Cav-1-positive vesicles in Mfsd2a+/+ and Mfsd2a−/− mice. n = 3 animals per genotype, 10 capillary cross-sections per animal. Data are mean ± s.e.m.; * P < 0.05 (Student’s t-test).