REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit polyclonal α-Mfsd2a	Cell Signaling Technologies	Under Development; Cat#Mfsd2a14-87; RRID:AB_2617168
Mouse monoclonal α-Claudin-5-488 conjugate	Thermo Fisher Scientific	Cat #352588; RRID:AB_2532189
Rat polyclonal α-PECAM	BD Biosciences	Cat #553370; RRID:AB_394816
Rabbit polyclonal α-Cav-1	Cell Signaling Technologies	Cat #3267; RRID:AB_2275453
Rabbit polyclonal α-NeuN	Millipore	Cat #MAB377; RRID:AB_2298772
Rabbit polyclonal α-VE-cadherin	Abcam	Cat #ab33168; RRID:AB_870662
Rabbit polyclonal α-PDGFR-β	Cell Signaling Technologies	Cat #3169; RRID:AB_2162497
Rabbit polyclonal α-Aquaporin-4	Millipore	Cat #ABN411; RRID:AB_2637077
Mouse monoclonal α-β-Actin	Sigma	Cat #A2228; RRID:AB_476697
Mouse monoclonal α-Tubulin	Sigma	Cat# T5168; RRID:AB_477579
Bacterial and Virus Strains
Biological Samples
Chemicals, Peptides, and Recombinant Proteins
Isolectin GS-IB4, from Griffonia simplicifolia	Thermo Fisher Scientific	Cat #I21411
10-kDa Dextran-tetramethylrhodamine conjugate	Thermo Fisher Scientific	Cat #D1817
Horseradish peroxidase, type II	Sigma-Aldrich	Cat #P8250-50KU
Cholera toxin subunit B, Alexa Fluor 555 conjugate	Thermo Fisher Scientific	Cat #C34776
PNA Bio Cas9 protein	Thermo Fisher Scientific	Cat #NC0789474
Critical Commercial Assays
Deposited Data
Experimental Models: Cell Lines
Human: HEK293T cells	ATCC	CRL-3216
Mouse: bEnd.3 cells	ATCC	CRL-2299
Human: PANC-1-MSFD2A-GFP	Dr. David Sabatini; (Reiling et al., 2011)	N/A
Experimental Models: Organisms/Strains
Mouse: Mfsd2a KO: B6;129S5-Mfsd2atm1Lex/Mmucd	Mouse Biology Program, University of California, Davis	MMRRC strain 032467-UCD; RRID: MGI:5755244
Mouse: Cav-1 KO: STOCK Cav1tm1Mls/J	Jackson Laboratory	JAX: 004585; RRID:IMSR_JAX:004585
Mouse: Mfsd2aD96A	This paper	N/A
Oligonucleotides
Mfsd2aD96A gRNA: GCCTTCACTGACCCTCTGGTGGG	Integrated DNA Technologies	N/A
Mfsd2aD96A Donor Oligonucleotide: TGCTTCTTCCCCATGGCAGGTGGAACCACTTCCTGCT TCCATTATCCTTTTTGTGGGCCGAGCCTGGGATGCCT TCACTGCCCCACTAGTGGGCTTCTGCATTAGCAAGTC CTCCTGGACCCGCCTGGGCCGCCTCATGCCCTGGTAA GTAGAGACGCTCCTTCGAAG	Integrated DNA Technologies	N/A
Genotyping Primers: Mfsd2aD96A: (Fwd): TTGGTGGTTCAGCTGTGCCA (Rev): GACACCAGAAGCCTGCCTTT (Sequencing): ATGCTCCGCTAAGGCAATGA	Integrated DNA Technologies	N/A
Genotyping Primers: Mfsd2a: (WT Fwd): CCTGGTTTGCTAAGTGCTAGC (WT Rev): GTTCACTGGCTTGGAGGATGC (KO Fwd): CACTTCCTAAAGCCTTACTTC (KO Rev): GCAGCGCATCGCCTTCTATC	Integrated DNA Technologies	N/A
Genotyping Primers: Cav1: (WT Fwd): GCACACCAAGGAGATTGACC (KO Fwd): CTCCAGACTGCCTTGGGAAAA (Rev): CTTGGCTGTCACCACACAC	Integrated DNA Technologies	N/A
Recombinant DNA
pCAG-GFP	Matsuda and Cepko, 2004	Addgene Plasmid #11150
pHAGE-PGK_IZsGreen	Harvard Medical School, Jeng-Shin Lee	https://plasmid.med.harvard.edu/PLASMID/GetVectorDetail.do?vectorid=251
Plasmid: pCAG-mMfsd2a-GFP	This paper	N/A
Plasmid: pHAGE-PGK_mMfsd2a-IZsGreen	This paper	N/A
Software and Algorithms
Prism 7	GraphPad	https://www.graphpad.com/scientificsoftware/prism/
CCTop – CRISPR/Cas9 target online predictor	Center for Organismal Studies Heidelberg; (Stemmer et al., 2015)	http://crispr.cos.uni-heidelberg.de/
TraceFinder 3.2	Thermo Fisher Scientific	http://tools.thermofisher.com/content/sfs/manuals/ Man-XCALI-97598-TraceFinder-32-User-Clinical-ManXCALI97598-A-EN.pdf
Custom ImageJ macro for quantifying BBB permeability	This paper	Data S1
Other