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. 2017 Jun 19;8:373. doi: 10.3389/fphar.2017.00373

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Copyright © 2017 Masuelli, Benvenuto, Mattera, Di Stefano, Zago, Taffera, Tresoldi, Giganti, Frajese, Berardi, Modesti and Bei.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of API on the autophagic flux in MM cells. The expression of Beclin-1, p62/SQSTM (p62), and LC3-I and LC3-II was assessed by Western blotting in MM cell lines treated with API at 50 μM or DMSO (CTR) for 24 h. Actin was used as an internal control. The intensity of the bands obtained was quantified using the ImageJ software after blot scanning of two independent experiments. The densitometric ratios between Beclin-1 and actin, LC3-I and actin, LC3-II and actin, p62 and actin and statistical analysis are reported. Data are expressed as the mean ± SD of two independent experiments (^∗p ≤ 0.01 compared with CTR).