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. 2017 Jun 19;8:373. doi: 10.3389/fphar.2017.00373

FIGURE 5.

FIGURE 5

Effect of API on the expression of molecules involved in apoptosis in MM cells. (A) The expression of Bax, Bcl-2, p53 was assessed by Western blotting analysis in MM cells treated for 24 h with API at 50 μM or with DMSO (CTR) as vehicle. Actin was used as an internal control. (B) The expression of procaspase 9, procaspase 8, cleaved caspase 8 fragments, and the cleavage of PARP-1 were assessed by Western blotting analysis in MM cells treated for 24 h with API at 50 μM or with DMSO (CTR) as vehicle. Actin was used as an internal control. The intensity of the bands was quantified using ImageJ software after blot scanning, obtained from two independent experiments. (C) The densitometric ratios between Bax and Bcl-2, between p53 and actin, and between procaspase 9 and actin and statistical analysis are reported. Data are expressed as the mean ± SD of two independent experiments (xp ≤ 0.05, ∗p ≤ 0.01 compared with CTR).