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Typical in vitro susceptibility testing workflow for Plasmodium falciparum. Synchronization of ring-stage intraerythrocytic parasites is required, followed by the addition of drugs to a microtiter plate. There are assays for culture-adapted laboratory strains as well as direct testing from patient blood. Parasitemia can be assessed in several ways, including by an ELISA or fluorescence via flow cytometry. There are several modifications of this basic format, including the RSA.
