Figure 2.

Early Peripheral A15/283 Treatment Provided Gender-Specific Amelioration of Tail Necrosis in C/C Mice

(A) Tail length over time for WT males injected with PBS (n = 9), CON15 (n = 17), and A15/283 (n = 23) (left) and WT females injected with PBS (n = 15), CON15 (n = 9), and A15/283 (n = 20) (right). (B) Tail length over time for C/C males injected with PBS (n = 9), CON15 (n = 11), and A15/283 (n = 7) (left) and C/C females injected with PBS (n = 11), CON15 (n = 14), and A15/283 (n = 12) (right). (C) Photograph of male and female WT and C/C tails at P60. Treatments are indicated above each column of tails. Scale bar, 25 mm. (D) Tail necrosis onset age for C/C mice. (E) Maximum tail length for C/C mice. (F) Ear necrosis onset age for C/C mice. (G) H&E-stained tail cross sections from male and female P60 mice injected with A15/283 at P1 and P3. The sex and genotype are indicated above each micrograph. Base refers to a cross section taken from the base of the tail near the body and middle refers to a cross section taken at the middle of the remaining tail stub. Micrographs with blood vessels show a higher magnification of the median caudal vasculature at the base of the tail. Black arrowhead indicates blood vessel muscle disorganization, and blue arrowhead indicates blood vessel obstruction. Base and middle micrographs scale bar, 200 μm; blood vessels micrographs scale bar, 50 μm. See also Figure S3 for tail micrographs from PBS-injected and CON15-treated mice. Error bars on graphs indicate SEM. For (A) and (B), data were analyzed with a two-way repeated-measures ANOVA followed by Tukey’s multiple comparison test. + indicates a significant difference between PBS and CON15, * indicates a significant difference between PBS and A15/283, and ˆ indicates a significant difference between CON15 and A15/283. For (D–F), data were analyzed by a two-way ANOVA followed by Tukey’s multiple comparison test. *p < 0.05 indicates a significant difference between the means.