. Author manuscript; available in PMC: 2018 Mar 1.

Published in final edited form as: J Neurochem. 2017 Jan 31;140(5):728–740. doi: 10.1111/jnc.13930

Table 1.

K_m values for DA uptake into CPu and NAc synaptosomes (by RDEV) and midbrain synaptoneurosomes (by [³H]DA) from rats on FR or OB diet

	DA Uptake (RDEV)		[³H]DA uptake
	CPu	NAc	Midbrain
	K_m (μM)	K_m (μM)	K_m (nM)

AL	3.15±0.15 (7)	3.83±0.36 (5)	30.5±0.67 (4)
FR	2.53±0.24 (10)	4.21±0.19 (6)	31.8±0.44 (5)
OB	2.32±0.26 (7)	2.76±0.15 (5)*	29.9±1.12 (4)

Absolute K_m values for DA uptake were determined over various concentrations of [DA] as measured by RDEV in freshly prepared CPu or NAc synaptosomes or synaptoneurosomes with [³H]DA prepared from frozen midbrain tissue. Kinetic constants were calculated using Kell Radlig and are presented as means ± SEM for the number of independent experiments indicated between parentheses. Each NAc experiment required tissue pooled from 2 rats (*p<0.05 vs. AL control, one-way ANOVA followed by Dunnett’s post hoc test).