Figure 3.

Glycerol and free fatty acid (FFA) release in cultured 3T3-L1 adipocytes incubated with rosmarinic acid (RA) in the presence or absence of wortmannin (wort, 100 nM), rolipram (rol, 5 μM), and cilostamide (cil, 5 μM) in Dulbecco’s modified Eagle’s medium supplemented with 2% fatty acid-free bovine serum albumin at 2 h (A), 24 h (B), and 48 h (C). Data are presented as means ± SEM. All cell culture experiments were repeated at least three times on two separate passages of cells (n = 6). *p < 0.05 vs vehicle (Veh) group at the same time-point; #p < 0.05 vs RA group at the same time-point.