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. 2017 Jun 7;61(1):1330096. doi: 10.1080/16546628.2017.1330096

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© 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Inhibition by rosmarinic acid (RA) of isoproterenol (ISO)- and forskolin (forsk)- stimulated glycerol and free fatty acid (FFA) release in cultured 3T3-L1 adipocytes. RA suppressed ISO- and forsk-stimulated glycerol and FFA release at both 2 h (A) and 24 h (B). RA inhibited ISO- and forsk-induced phosphorylation of hormone-sensitive lipase (HSL)ser660 and perilipin A (C). Representative blots are shown to the right of the graphs in (C). Data are presented as means ± SEM. All cell culture experiments were repeated at least three times on two separate passages of cells (n = 6). *p < 0.05 vs vehicle (Veh) group at the same time-point; #p < 0.05 vs RA group at the same time-point.