Table 1.
Orthogonal analysis of CTMS labelling (*p < 0.05, **p < 0.01) including the factors and levels (A), the arrangement and results of orthogonal experiments (B), and the variance analysis (C).
| Levels | Factors |
|||
|---|---|---|---|---|
| (A) pH | (B) Temperature (°C) | (C) Ratio of CTMS:FITC | (D) Concentration of CTMS (mg/ml) | |
| A | ||||
| 1 | 5.5 | 15 | 100:1.5 | 1 |
| 2 | 7.5 | 25 | 100:3 | 1.5 |
| 3 | 9.5 | 35 | 100:4.5 | 2 |
| No. |
A |
B |
C |
D |
Label efficiency (%) |
|---|---|---|---|---|---|
| B | |||||
| 1 | 1 | 1 | 1 | 1 | 0.05 |
| 2 | 1 | 2 | 2 | 2 | 0.43 |
| 3 | 1 | 3 | 3 | 3 | 1.92 |
| 4 | 2 | 1 | 2 | 3 | 1.49 |
| 5 | 2 | 2 | 3 | 1 | 2.65 |
| 6 | 2 | 3 | 1 | 2 | 0.60 |
| 7 | 3 | 1 | 3 | 2 | 0.97 |
| 8 | 3 | 2 | 1 | 3 | 0.75 |
| 9 | 3 | 3 | 2 | 1 | 0.34 |
| K1 | 0.80 | 0.84 | 0.47 | 1.01 | |
| K2 | 1.58 | 1.28 | 0.75 | 0.68 | |
| K3 | 0.69 | 0.95 | 1.85 | 1.39 | |
| R | 0.89 | 0.44 | 1.38 | 0.72 | |
| Factors |
SS |
f |
F |
F* |
Significance |
|---|---|---|---|---|---|
| C | |||||
| A | 4.04 | 2 | 2.02 | 518.44 | ** |
| B | 1.09 | 2 | 0.54 | 139.68 | * |
| C | 9.89 | 2 | 4.94 | 1268.5 | ** |
| D | 2.29 | 2 | 1.15 | 294.03 | * |