Figure 5.

Levels of defense hormones are not differentially impacted by G. orontii inoculation in mlo2 mlo6 mlo12 vs. Col-0 but SA accumulates in mlo2 mlo6 mlo12 independently of an infection. Five to six-week-old plants of indicated genotypes were leaf-to-leaf inoculated with G. orontii, mock-inoculated, treated with 1 μM nlp24 (ET), wounded (JA), or kept as non-inoculated control plants. At 12 hpi, 3 h post nlp24 treatment or 30 min after wounding leaves were sampled and hormones were measured by GC (ET) or HPLC-MS/MS (JA, SA, SAG). Data represent means of 2 (ET) or 6 (JA, SA, SAG) biological replicates +/− SE. Distinct lower-case letters indicate statistically significant differences between groups as determined by GLM (P ≤ 0.05).