Figure 8.

Accumulation of 4MI3G upon inoculation depends on the presence of MLO2, MLO6 and MLO12 during interaction with an adapted, but not with a non-adapted powdery mildew fungus. Four to five-week-old plants of the indicated genotypes were inoculated with G. orontii, B. graminis f. sp. hordei, or kept as non-inoculated control plants. Leaves were sampled at the indicated time points after inoculation, metabolites extracted and compound concentrations measured by HPLC. Data represent means of 3 independent biological replicates ± SE. Distinct lower-case letters indicate statistically significant differences between groups as determined by GLM (P ≤ 0.05).