Table 1.
Transcript levels of JA/ET-responsive genes are elevated in mlo2 mlo6 mlo12 mutant plants in response to inoculation with the powdery mildew fungus G. orontii.
| Arabidopsis Genome ID | Gene annotation and putative function of encoded protein | Fold change mlo2 mlo6 mlo12/Col-0 G. orontii | Induction of gene expression in Col-0 in response to a | ||
|---|---|---|---|---|---|
| 8 hpi | 12 hpi | JA | JA + ET | ||
| AT5G44420 | PDF1.2a (Plant Defensin 1.2a) | 5.0 | 6.3 | yes | yes |
| AT5G61160 | AACT1 (Anthocyanin-5-Aromatic Acyltransferase 1) | 4.9 | 6.2 | yes | yes |
| AT3G49620 | DIN11 (Dark Inducible 11) | 4.7 | 7.3 | yes | yes |
| AT3G04720 | PR4 (Pathogenesis-Related Protein 4) | 4.3 | 2.8 | - | yes |
| AT4G16260 | Glycosyl hydrolase family 17 protein | 4.0 | 5.8 | - | yes |
| AT2G26020 | PDF1.2b (Plant Defensin 1.2b) | 3.9 | 7.1 | yes | yes |
| AT2G26560 | PLP2 (Phospholipase A 2A) | 3.5 | - | yes | yes |
| AT1G67810 | Fe-S metabolism associated domain-containing protein | 3.5 | 2.7 | - | yes |
| AT3G16530 | Lectin like protein | 3.2 | 2.2 | yes | yes |
| AT1G26380 | FAD-binding domain-containing protein | 2.9 | - | yes | yes |
| AT5G25250 | Unknown protein | 2.8 | 2.2 | - | yes |
| AT1G17745 | PGDH (3-Phosphoglycerate Dehydrogenase) | 2.7 | 2.5 | yes | yes |
| AT3G28930 | AIG2, avrRpt2-induced gene | 2.6 | 1.7 | yes | yes |
| AT2G38860 | Protease I (pfpI)-like protein YLS5 | 2.5 | 2.0 | - | yes |
| AT1G06160 | ORA59 (AP2/ERF domain transcription factor) | 2.4 | 2.8 | - | yes |
| AT1G02920 | GST7 (Glutathione-S-Transferase 7) | 2.4 | - | yes | yes |
| AT2G04400 | IGPS (Indole-3-Glycerol Phosphate Synthase) | 2.3 | 2.0 | - | yes |
| AT5G47220 | ERF2 (AP2/ERF domain transcription factor) | 2.2 | 1.9 | yesb | yesb |
| AT5G10520 | RBK1 (ROP binding protein kinases 1) | - | 3.8 | - | yes |
| AT1G30135 | JAZ8 (Jasmonate-Zim-domain protein 8) | - | 2.7 | yesb | -b |
| AT1G07260 | UGT71C3 (UDP-glycosyltransferase 71C3) | 1.3 | 2.6 | - | yes |
| AT4G08770 | Peroxidase, putative | 1.7 | 2.6 | yes | yes |
| AT4G24350 | Phosphorylase family protein | - | 2.3 | yes | yes |
| AT1G51760 | JR3 (Jasmonic Acid Responsive 3) | - | 2.3 | yesb | -b |
| AT4G17500 | ERF1 (AP2/ERF domain transcription factor 1) | - | 2.2 | yesb | yesb |
| AT4G24340 | Phosphorylase family protein | - | 2.3 | yes | yes |
| AT5G54960 | PDC2 (Pyruvate Decarboxylase 2) | - | 2.2 | - | yes |
| AT1G17380 | JAZ5 (Jasmonate-Zim-domain protein 5) | - | 2.2 | yesb | -b |
| AT4G34200 | EDA9 (Embryo Sac Development Arrest 9) | - | 2.0 | yes | yes |
a
Genes induced in Col-0 wild type plants after treatment with JA or JA and ET (Pré et al., 2008).
b
These genes were characterized using Genevestigator V3 (Hruz et al., 2008).
Genes were identified by comparative transcriptome analysis upon G. orontii challenge and manually selected from candidate genes with a statistically significant (P ≤ 0.05) and at least two-fold change in transcript abundance in the mlo2-5 mlo6-2 mlo12-1 mutant compared with Col-0 wild type (Table S1). This gene selection was compared with published expression data from Col-0 plants treated with JA or JA and ET.