Table 1. Bile acid exposure increased Fxr and decreased Atg7 expression.

Fxr	Control n=3		GCDC=3
		%		%	P value
Total cells	15 348	100	14 345	100
Fxr	6247	40.7	9208	64.2	0.0433
Fxr	GS n=3		GCDC+GS=3
		%		%	P value
Total cells	8937	100	11 018	100
Fxr	3488	39	3498	31.7	ns
Fxr	Control n=3		TCA=3
		%		%	P value
Total cells	12 658	100	8361	100
Fxr	5487	43.3	4496	53.8	0.0107
Fxr	GS n=3		TCA+GS=3
		%		%	P value
Total cells	9740	100	5279	100
Fxr	5033	51.6	2614	49.5	ns
Atg7	Control n=3		GCDC=3
		%		%	P value
Total cells	10 245	100	9137	100
Fxr	4163	40.6	2465	27	0.0497
Atg7	GS n=3		GCDC+GS=3
		%		%	P value
Total cells	8450	100	6565	100
Fxr	3180	37.6	2966	45.1	0.0004
Atg7	Control n=3		TCA=3
		%		%	P value
Total cells	17 467	100	11 984	100
Fxr	7676	43.9	4102	34.2	0.0661
Atg7	GS n=3		TCA+GS=3
		%		%	P value
Total cells	15 611	100	11 136	100
Fxr	4359	27.9	5350	48	ns

Table 1 summarizes the number of positive stained cells for Fxr or Atg7 in response to GCDC or TCA with or without GS for three independent experiments. Cell numbers were identified and count using StataQuest analysis software (TissueGnostics), and expressed as % as described in the methods.